使用自动化分子检测系统进行例行母乳监测,减少了早产婴儿产后的CMV感染
Junhyup Song1, Sinyoung Kim1, Eunmin Kwak1
1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
Frontiers in microbiology
|October 5, 2023
概括
一种新方法可以在母乳中可靠地检测人类细胞巨乳病毒 (CMV),这对于预防早产婴儿的致命风险至关重要. 这种方法有助于临床医生监测CMV阳性并减少传播.
科学领域:
- 病毒学 病毒学
- 新生儿医学 新生儿医学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 母乳中的人类细胞巨乳病毒 (CMV) 对早产婴儿构成重大风险.
- 现有的分子测试通常不适合母乳矩阵.
研究的目的:
- 评估Cobas CMV测试在Cobas 6,800系统上的表现,用于在母乳中检测人类细胞巨病毒 (CMV).
- 建立一种可靠的方法来监测母乳中的CMV,以确保早产婴儿的安全.
主要方法:
- 为母乳开发了一种简单,独立的样本制备程序.
- 在Cobas 6,800系统上使用了自动化的Cobas CMV测定.
- 评估了分析性能 (线性) 和临床性能 (阳性率,传播率).
主要成果:
- 母乳中CMV阳性率为57.8%,新生儿为5.2%,传播率为7.7%.
- 与传统方法相比,简化样品制备方法显示出同等或更好的灵敏度.
- 在1.87-4.73 log IU/mL范围内,线性得到证实.
结论:
- 开发的方法有效地使用自动化系统在母乳中检测CMV.
- 这种方法为临床医生提供了一种方便可靠的工具来监测母乳CMV,有助于减少垂直传播.
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