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裸体Endosperm1,裸体Endosperm2和OPAQUE2相互作用,调节玉米内发育中的基因网络

Hao Wu¹, Mary Galli², Carla J Spears³

¹Genetics, Development and Cell Biology Department, Iowa State University, Ames, IA 50011, USA.

|October 5, 2023

概括

玉米内的发展涉及NAKED ENDOSPERM1 (NKD1),NKD2和OPAQUE2 (O2) 转录因子. 这些因素动态调节基因网络,影响谷物填充期间的细胞模式和营养储存.

科学领域:

植物分子生物学植物分子生物学
玉米的遗传学玉米的遗传学
转录条例转录条例转录条例

背景情况:

裸体内1 (NKD1),NKD2和OPAQUE2 (O2) 是玉米 (Zea mays) 内中的关键转录因子.
这些因素对于细胞模式和营养储存至关重要,但它们的复杂调控相互关系尚未完全理解.

研究的目的:

研究NKD1,NKD2和O2在共同调节基因网络中的复杂调节相互作用.
阐明它们在从细胞发育过渡到玉米内的营养积累过程中的动态作用.

主要方法:

对nkd1,nkd2和o2突变体和野生类型基因的所有组合的同卵性基因的开发.
多种基因型的表型分析.
用测序 (ATAC-seq) 进行基因网络分析和转移酶可访问染色质的测试.

主要成果:

在8种基因型中观察到影响细胞模式,粉和储存蛋白质的多种表型和基因相互作用.
NKD1,NKD2和O2动态调节层次基因网络,从指导细胞发育转向促进营养生物合成和储存.
氧2降低了NKD1/NKD2目标地点的可访问性,而NKD1/NKD2增加了O2目标地点的可访问性,这表明了动态的监管景观控制.

结论:

NKD1,NKD2和O2的相互作用动态地塑造了层次性的基因网络和监管格局.
这些转录因子协调了从细胞发育到玉米内的谷物填充的关键过渡.

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