乳酸受体的激活积极调节小鼠骨肌肉质量
1Faculty of Rehabilitation and Care, Seijoh University, Tokai, Japan. gotok@sepia.ocn.ne.jp.
Physiological research
|October 5, 2023
概括
乳酸受体G蛋白结合受体81 (GPR81) 的激活刺激了骨肌肉的生长. 口服GPR81激动剂增加了小鼠的肌肉质量和蛋白质含量,这表明它在肌肉缩中发挥了作用.
科学领域:
- 生物化学 生物化学
- 生理学 生理学 生理学
- 分子生物学分子生物学
背景情况:
- G蛋白结合受体81 (GPR81) 是一种乳酸受体,存在于骨肌肉中.
- 在骨肌中GPR81的确切生理功能尚不清楚.
- 乳酸盐的使用与肌肉缩有关,这表明GPR81的参与.
研究的目的:
- 研究GPR81激活在骨肌肉质量调节中的作用.
- 为了确定GPR81激动剂的使用是否可以在体内诱导骨肌肉缩.
主要方法:
- 雄性C57BL/6J小鼠接受了特定的GPR81激动剂 (3-Chloro-5-hydroxybenzoic acid,CHBA) 或对照剂的治疗.
- 在快速抽的肌肉 (植物肌) 和缓慢抽的肌肉 (肌底肌) 中证实了GPR81蛋白表达.
- 评估了肌肉体重,蛋白质含量和信号通路激活 (ERK1/2,p90RSK).
主要成果:
- 施用CHBA显著增加了植物肌和肌肉肌的绝对和相对肌肉重量.
- 在CHBA治疗后,绝对和相对肌肉蛋白含量都显著增加.
- CHBA上调了细胞外信号调节激酶-1/2 (ERK1/2) 和p90核糖体S6激酶 (p90RSK) 的酸化.
结论:
- 在骨肌中激活GPR81刺激肌肉质量在体内增加.
- 该ERK信号通路可能参与调解GPR81激活的过度缩效应.
- GPR81代表了增强骨肌肉生长的潜在治疗标.
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