主体血管新生素和登革热病毒复制之间的协同相关性
Deeksha Madhry1, Shivani Malvankar1, Sushant Phadnis1
1Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, India.
RNA biology
|October 5, 2023
概括
登革热病毒 (DENV) 感染会增加宿主细胞中的压力调节剂 ангиогенин. 用伊弗梅克丁抑制血管新生素减少了DENV的复制,这表明它在病毒病变发生过程中的作用.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 细胞应激反应的应激反应
背景情况:
- 登革热病毒 (DENV) 感染是全球健康的威胁.
- DENV 病原发生在很大程度上依赖于宿主细胞机械.
- 对于DENV与主机相互作用的机制细节的描述仍然不完全.
研究的目的:
- 描述DENV诱导宿主细胞应激的机制基础.
- 为了研究压力调节器核糖核糖酶 (Ribonuclease Angiogenin) 在DENV感染中的作用.
主要方法:
- 在DENV感染细胞中量化血管新生素水平.
- 在DENV感染和模拟感染细胞中,siRNA介导的血管新生素的淘汰.
- 在DENV感染之前用Ivermectin治疗Huh7细胞.
主要成果:
- 在DENV感染的细胞中,血管新生素水平上调,随着病毒复制而增加.
- 在DENV感染的细胞中,siRNA对血管新生素的淘汰没有成功.
- 艾弗梅克丁治疗显著降低了DENV复制和血管新生素水平在后期阶段.
结论:
- 血管新生素的上调与DENV复制有关.
- 血管原蛋白调节在DENV复制和病变发生中起着至关重要的作用.
- 向血管新生素可能提供针对DENV的治疗策略.
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