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miR156/SPL12模块通过直接调节蓝中的乙烯生产途径来调节水果颜色的变化

Hongxue Li¹, Shouwen Wang¹, Lulu Zhai¹

¹College of Plant Science, Jilin University, Changchun, China.

Plant biotechnology journal

|October 5, 2023

概括

蓝果的颜色变化涉及微RNA156/SPLs和乙烯. 这项研究揭示了VcMIR156a和VcSPL12调节乙烯生产和色素积累,连接这些途径.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

果实成熟过程涉及颜色的变化,由素和叶绿素水平介导.
微RNA156/SPLs和乙烯是已知的果实成熟的调节剂,但它们的相互作用尚不清楚.
之前的研究表明,蓝VcMIR156a/VcSPL12对模型植物的色素积累产生影响.

研究的目的:

为了研究miR156/SPL模块与蓝果色变化中的乙烯通路之间的内在联系.
阐明VcMIR156a和VcSPL12在调节素,叶绿素和乙烯生物合成中的作用.
在这些过程中识别VcMIR156a和VcSPL12的分子标.

主要方法:

在蓝水果中过度表达VcMIR156a和VcSPL12.
测量圣素和叶绿素含量.
对乙烯生物合成基因表达和ACC水平的分析.
用乙烯替代品和抑制剂进行处理.
蛋白质-DNA相互作用试验 (例如,ChIP-qPCR).

主要成果:

过度表达VcMIR156a增加了安东素,减少了叶绿素,提高了乙烯生物合成基因表达和ACC水平.
过度表达VcSPL12增强了叶绿素,抑制了阳素,并降低了ACC合成.
乙烯操纵改变了VcMIR156a和VcSPL12对色素积累的影响.
VcSPL12直接向和抑制了乙烯生物合成基因VcACS1和VcACO6.6,这些基因是乙烯生物合成的直接向和抑制.
VcSPL12可能与VcDVR促进体相互作用,激活叶绿素生物合成.

结论:

VcMIR156a/VcSPL12模块通过准VcACS1和VcACO6.6来调节乙烯生产,从而调节蓝果的颜色.
VcSPL12也在叶绿素积累中发挥作用,可能是通过与VcDVR的直接相互作用.
这项研究确定了miR156/SPL途径与水果成熟过程中的乙烯信号之间的直接联系.

关键词:

这就是VcMIR156a.在VcSPL12中使用.它们是安托西安氨酸.蓝蓝蓝是一种叶绿素的含量是多少叶绿素的含量是多少乙烯乙烯的使用情况

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