卵巢中的母细胞动力学由GnRH和益生菌控制
Titaree Laoharatchatathanin1,2, Duangjai Rieanrakwong1, Yoshinori Hatsugai1
1Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Towada 034-8628, Japan.
淋巴激素释放激素 (GnRH) 积极调节卵巢巨细胞 (MC) 数量,而益生菌则对它们产生负面影响. 这突显了卵巢功能和免疫细胞动态中的新型荷尔蒙相互作用.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 已知释放性激素 (GnRH) 能够影响乳腺中巨细胞 (MC) 的迁移.
- 此外,GnRH也在卵巢中表达,这表明它可能在卵巢免疫细胞调节中发挥作用.
- 乳腺细胞是存在于包括卵巢在内的各种组织中的免疫细胞,在炎症和组织重塑中发挥作用.
研究的目的:
- 为了研究GnRH在调节卵巢内的巨细胞种群中的作用.
- 检查雌性周期和怀孕对卵巢巨细胞数量和GnRH表达的影响.
- 确定益生素和多巴胺对卵巢巨细胞的影响以及它们与GnRH的关系.
主要方法:
- 在雌性周期期间,对卵巢组织中乳腺细胞数量和分布的分析.
- 激光微解剖和定量实时PCR用于评估卵巢区的GnRH mRNA表达.
- 免疫组织化学可视化GnRH免疫活性和巨细胞染色.
- 在体内和体外 (特朗斯韦尔试验) 局部施用GnRH激动剂 (GnRHa) 和益生素/多巴胺激动剂/对抗剂.
主要成果:
- 卵巢巨细胞数量呈现周期性波动,在期和前期出现峰值.
- 在整个卵巢组织中检测到了GnRH mRNA和免疫活性,而巨细胞显示出强大的GnRH免疫染.
- 卵巢GnRHmRNA水平与雌性周期期间的巨细胞数量相关.
- 局部GnRH激动剂的使用增加了卵巢质细胞,而怀孕降低了质细胞和GnRH mRNA.
- 普罗拉克丁抑制了卵巢巨细胞峰值,并抑制了GnRHa诱导的腹膜巨细胞迁移.
- 一种多巴胺激动剂增加了卵巢巨细胞,当在伪怀孕期间益生菌水平升高时.
结论:
- 卵巢巨细胞数量受到局部GnRH表达的积极调节.
- 益生菌对卵巢GNRH和巨细胞数量产生负面调节作用.
- 益生菌对GnRH和巨细胞的抑制作用可能导致其在怀孕期间的黄质营养作用.
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