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在多微生物细菌病的血液培养鉴定 (BCID) 性能

William S Bradford¹, Monica A Donnelley², Jeffrey R Fine³

¹Division of Hospital Medicine, Department of Internal Medicine, UC Davis Medical Center, Sacramento, CA, USA.

Diagnostic microbiology and infectious disease

|October 5, 2023

概括

在FilmArray®血液培养识别 (BCID) 试验,一个快速多重PCR (rmPCR) 试验,经常错过了多微生物细菌病 (PMB) 的生物体. 这种不完整的识别影响了这种常见疾病的诊断准确性.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
分子诊断学分子诊断学

背景情况:

基于快速多重PCR (rmPCR) 的FilmArray®血液培养识别 (BCID) 试验显著减少了从阳性血液培养物中识别生物体的时间.
多微生物细菌性细菌病 (PMB) 对BCID检测提出了挑战,诊断准确性的已知局限性需要进一步表征.

研究的目的:

评估FilmArray® BCID测定在临床确认的多微生物细菌病例中的诊断性能.
通过使用FilmArray® BCID测定,确定PMB中不完全识别生物体的频率.

主要方法:

在23个月的时间里,在一个学术中心对临床确认的PMB病例进行了回顾性队列分析.
通过FilmArray® BCID测定对207个已识别和研究的样本进行了评估,以进行完全或不完全的识别.

主要成果:

几乎一半 (49.3%) 的多微生物培养被FilmArray® BCID测定不完全识别.
在患者的并发病,住院时间,死亡率或感染源方面,在完全或不完全识别的组之间没有观察到显著的差异.
该研究强调了在使用基于rmPCR的测定时,经常低估PMB中的微生物存在.

结论:

基于rmPCR的测试,如FilmArray® BCID,经常无法识别多微生物细菌病的所有致病生物.
在PMB病例中FilmArray® BCID结果的临床解释应该考虑潜在的病原体不充分识别.
可能需要进一步的研究来提高分子诊断工具对多微生物感染的敏感性.

关键词:

这是一个BCIDBCIDBCID.细菌病是什么意思细菌病是什么意思电影集电影集电影集多微生物多样化

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