使用塑料和不钢柱子进行超声波辅助色谱的机械研究
Nguyen Van Kien1, Young Han Jeong1, David Jin Han Seog1
1Department of Chemistry Education, Kyungpook National University, Daegu, 702-701, the Republic of Korea South Korea.
Journal of chromatography. A
|October 5, 2023
概括
超声波辅助染色学 (UAC) 显示了塑料和不钢柱的不同机制. 超声波很容易穿透塑料柱子,导致洞穴形成并影响分离,而不钢柱子显示的超声波影响很小,主要是温度变化.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 物理化学 物理化学
背景情况:
- 之前的研究探讨了使用塑料和不钢 (SS) 柱子的超声波辅助染色学 (UAC).
- 精确的UAC机制,特别是关于柱子材料差异,需要进一步阐明.
- 了解UAC机制对于优化聚环芳 (PAH) 分离技术至关重要.
研究的目的:
- 在UAC中对塑料 (PEEK) 和不钢 (SS) 柱子的超声波效应进行比较分析.
- 通过使用C18静止相来分离多环芳 (PAHs) 的UAC机制.
- 为UAC分离机制建立一个彻底的解释,包括奇拉和尺寸排除色谱.
主要方法:
- 对使用C18静止相的PEEK和SS柱的超声波效应进行比较分析.
- 在各种超声强度下 (0-100%的900W) 分离多环芳 (PAHs) 的混合物.
- 测量H值 (板高度) 和保留时间,并估计温度变化.
主要成果:
- 在超声波下,在PEEK和SS柱之间观察到H值的显著差异.
- 超声波柱显示H值的最小增加 (pyrene的8.829.86μm) 与超声波强度的增加,表明超声波透性差.
- 由于声洞化,PEEK柱体的H值增加了12倍 (11.5134μm对于烯),导致保留时间减少 (2-9%) 和峰值扭曲.
结论:
- 超声波能量透率在PEEK和SS柱之间有很大的差异.
- 在SS柱中,温度升高是PAH分离的主要因素,而在PEEK柱中,声波化占主导地位.
- 该研究提供了一个全面的解释UAC分离机制在各种染色学模式.
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