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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift.

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Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states.

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Multifaceted effects of N-glycosylation on amyloidogenic κ light chains in AL amyloidosis.

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Near-complete cryo-EM structure of the Klebsiella pneumoniae podophage RAN69 reveals tail fiber-spike interface and a divergent pre-ejectosome.

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相关实验视频

Updated: Jul 14, 2025

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
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Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

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病毒结构通过先进的冷电子显微镜方法揭示.

Dongjie Zhu1, Duanfang Cao2, Xinzheng Zhang2

  • 1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

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|October 5, 2023
PubMed
概括
此摘要是机器生成的。

先进的冷电子显微镜 (cryo-EM) 方法使高分辨率的病毒结构确定成为可能. 本综述涵盖了诸如单粒子分析 (SPA) 和冷电子断层扫描 (cryo-ET) 等技术,以了解病毒结构和动态.

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
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Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

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相关实验视频

Last Updated: Jul 14, 2025

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Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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科学领域:

  • 结构生物学 结构生物学
  • 病毒学 病毒学
  • 显微镜的使用方法

背景情况:

  • 电子显微镜 (cryo-EM) 单颗粒分析 (SPA) 历史上实现了对称病毒的高分辨率.
  • 病毒结构多样化,并经历着形状变化,需要先进的成像技术.

研究的目的:

  • 审查用于高分辨率病毒结构确定的先进的冷EM方法.
  • 总结利用冷EM进行病毒结构分析所取得的成就,并讨论病毒结构分析的挑战.

主要方法:

  • 不对称的重建重建.
  • 埃瓦尔德球的纠正 球的纠正
  • 低温电子断层扫描 (cryo-ET) 是一种电子断层扫描.
  • 亚断层图谱平均值 (STA) 的平均值

主要成果:

  • 已经成功地应用了冷EM方法来确定复杂的病毒结构.
  • 先进的技术允许对病毒进行体内和体外结构分析.

结论:

  • 当前先进的冷EM方法为高分辨率的病毒结构确定提供了强大的工具.
  • 解决持续存在的挑战将进一步提高我们对病毒结构和功能的理解.