德尤比基提纳酶UCHL1通过稳定电压依赖的阴离子通道2来调节雌激醇合成
Shengjie Shi1, Guiyan Chu1, Lutong Zhang1
1College of Animal Science and Technology, Northwest A&F University, Yangling, China; Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling, China.
The Journal of biological chemistry
|October 5, 2023
概括
乌比奎丁C终端酶1 (UCHL1) 通过稳定VDAC2促进雌激素合成,这对母猪和小鼠的毛囊发育和生殖性能至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 乌比奎丁生物学
背景情况:
- 颗粒细胞的雌激素生产对卵泡发育和排卵至关重要.
- 无素-蛋白酶体系统调节蛋白质平衡,影响雌性循环.
- 杜比基化酶在雌激醇合成中的作用在很大程度上仍未被探索.
研究的目的:
- 调查duebiquitinase UCHL1在雌激醇合成中的功能及其对生殖能力的影响.
主要方法:
- 在不同生殖状态的母猪中对UCHL1表达的比较分析.
- 在体外研究涉及UCHL1过度表达和干扰颗粒细胞.
- 生物化学测试以确定UCHL1-VDAC2相互作用和无处不在的位.
- 在小鼠体内实验中使用AAV介导的基因传递来评估UCHL1对雌性周期和卵巢功能的影响.
主要成果:
- 在雌性和高大小母猪中,UCHL1的表达更高.
- UCHL1的过度表达增强了雌激素的合成,而UCHL1的干扰则减少了它.
- UCHL1与VDAC2结合,双化,稳定VDAC2,这是雌激醇前体合成的关键调节器.
- 在体内,UCHL1 Knockdown 干扰雌激素循环,降低雌激醇水平,并减少小鼠中 antral 毛囊的数量.
结论:
- UCHL1通过稳定VDAC2促进雌激醇合成,从而支持卵泡发育和生殖能力.
- UCHL1被确定为影响生殖表现的重要因素和潜在的治疗点.
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