在Tolypocladium专辑中的离散的Indole Terpenoid基因集群之间的功能交叉
Taylor R Hibbard1,2, Rose M McLellan1,2, Luke J Stevenson1,2
1Ferrier Research Institute, Victoria University of Wellington, Wellington 6012, New Zealand.
Organic letters
|October 5, 2023
概括
丝状真菌可以产生类. 托利波克拉专辑独特地包含了印二烯 (IDT) 和印六烯 (IST) 的通路,SES集群量身定制了IDT基质.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 自然产品 化学 化学
背景情况:
- 印度醇类是具有显著生物活性的多样化的二次代谢产物.
- 在真菌中,Indole diterpene (IDT) 和Indole sesquiterpene (IST) 生物合成途径通常分别存在.
- 丝状真菌Tolypocladium album是新型印类类物质的潜在来源.
研究的目的:
- 为了调查 IDT 和 IST 途径在 Tolypocladium album 中的同时发生.
- 阐明SES基因集群在类类生物合成中的功能.
- 探索醇类的代谢工程的潜力.
主要方法:
- 对Tolypocladium专辑进行基因组分析,以确定相关的基因集群.
- 异质表达和SES基因集群的重建.
- 生物化学测试以表征SES集群产品的酶活性.
主要成果:
- 托利波克拉基因组编码了IDT (TER集群) 和IST (SES集群) 路径.
- 异质重建证实SES星团负责IST生物合成.
- 该SES集群证明了其修改TER集群产生的IDT前体的能力.
结论:
- 托利波克拉专辑具有独特的双通道,用于类类生物合成.
- 该SES集群在IST生产中发挥着至关重要的作用,并可以影响IDT代谢.
- 这一发现为发现和设计新型的内醇化物开辟了道路.
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