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相关概念视频

Tight Junctions01:29

Tight Junctions

5.3K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.3K
Adherens Junctions01:24

Adherens Junctions

4.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
4.8K
Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

25.6K
The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...
25.6K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.7K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.7K
Anchoring Junctions01:03

Anchoring Junctions

3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.7K

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相关实验视频

Updated: Jul 14, 2025

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
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Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

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克劳丁-23重塑了表皮紧结架构,以调节屏障功能.

Arturo Raya-Sandino1, Kristen M Lozada-Soto1, Nandhini Rajagopal2

  • 1Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA.

Nature communications
|October 5, 2023
PubMed
概括

克劳丁-23 (CLDN23) 通过与CLDN3和CLDN4.4形成复合物来加强肠道上皮屏障. 这些蛋白质相互作用产生独特的紧密结合孔,调节透性和屏障功能.

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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

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相关实验视频

Last Updated: Jul 14, 2025

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
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科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 胃肠病学 胃肠病学

背景情况:

  • 紧结蛋白,克劳丁,调节上皮质屏障的功能.
  • 胃肠道屏障功能的异质性与差异化的克劳丁表达有关.

研究的目的:

  • 研究克劳丁-23 (CLDN23) 在肠上皮质屏障功能中的作用.
  • 阐明CLDN23调节细胞透性的机制.

主要方法:

  • 利用互补的实验方法来研究CLDN23的功能.
  • 采用计算建模来分析克劳丁复合物形成和孔隙结构.

主要成果:

  • CLDN23富含在光道肠上皮细胞中,增强了屏障完整性.
  • CLDN23与CLDN3和CLDN4结合,影响它们的局部化和调节离子/巨分子透性.
  • 计算模型显示,CLDN23-CLDN3/CLDN4复合体形成独特的孔隙,具有不同的电荷和结构.

结论:

  • 通过与CLDN3和CLDN4.4相互作用,CLDN23加强了上皮屏障.
  • 克劳丁复合体的形成依赖于相互作用,导致不同的孔状性质.
  • 提出了一个模型,其中不同的克劳丁复合体通过改变的紧密结结构来调节上皮质屏障功能.