在COPD唾液样本中对疫苗候选基因的ex-vivoRNA表达分析
Cecilia Brettoni1, Alessandro Muzzi2, Simona Rondini2
1GSK, Via Fiorentina 1, Siena, Italy. cecilia.x.brettoni@gsk.com.
Respiratory research
|October 5, 2023
概括
一种针对常见的COPD恶化细菌的疫苗,非类型的Haemophilus influenzae (NTHi) 和Moraxella catarrhalis (Mcat),在COPD患者中显示出可变的基因表达. 基因表达水平在稳定状态和恶化状态之间没有显著差异.
科学领域:
- 呼吸系统医学 呼吸系统医学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 慢性阻塞性肺病 (COPD) 恶化显著增加了发病率和死亡率.
- 细菌感染,特别是非类型的Haemophilus influenzae (NTHi) 和Moraxella catarrhalis (Mcat),是COPD恶化的主要触发因素.
- 一种针对NTHi和Mcat的疫苗已开发并在临床试验中进行评估.
研究的目的:
- 评估COPD患者唾液中关键NTHi和Mcat基因的体内表达.
- 为了确定特定NTHi和Mcat疫苗候选基因的表达是否在稳定的COPD和恶化期间有所不同.
- 探索基因表达,COPD严重程度和恶化频率之间的潜在相关性.
主要方法:
- 一项前性,观察性队列研究,在两年内涉及123名COPD患者.
- 在稳定期和急性恶化期间,从患者身上采集了唾液样本.
- 用RT-PCR分析选定NTHi和Mcat基因的mRNA表达.
主要成果:
- 来自NTHi和Mcat的疫苗候选基因在唾液中表达,表明体内存在和活性.
- 在稳定状态和恶化状态之间没有观察到NTHi (pd,pe,pilA) 和Mcat (uspA2) 疫苗候选基因表达的统计学上显著差异.
- 在NTHi基因表达 (pd,pilA) 和先前恶化史,以及Mcat基因 (uspA2) 与COPD严重程度之间发现了轻微的关联.
结论:
- NTHi-Mcat疫苗候选基因在COPD患者的唾液中得到表达.
- 候选疫苗的基因表达水平在稳定阶段和恶化阶段之间没有显著差异.
- 虽然没有统计学意义,但一些基因表达模式可能与COPD恶化史和疾病严重程度相关.
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