探索一种Streptomyces的合成酶,使用乙烯-SNACs作为捐赠基质
Federica Casolari1, Saad Alrashdi1,2, Reuben Carr3
1Department of Chemistry, University of Aberdeen Aberdeen AB24 3UE UK h.deng@abdn.ac.uk.
RSC chemical biology
|October 6, 2023
概括
研究人员从Streptomyces coelicolor中设计了一种细菌合成酶 (WS/DGAT),以产生有价值的短/分支 Ester. 一种经过修改的酶变体L25F显示,对多种不同基捐赠体的催化效率有所提高.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 工业生物技术 工业生物技术
- 微生物生物化学 微生物生物化学
背景情况:
- Ester 是香水和食品行业的有价值化合物,对短/分支类型的需求日益增加.
- 合成酶/二甲糖醇O-转移酶 (WS/DGATs) 是一种细菌酶,可催化 Ester 的合成.
- 目前生产特定 Ester 的方法在效率和基质应用方面存在局限性.
研究的目的:
- 研究一种Streptomyces coelicolor WS/DGAT在生产短/分支 Ester 的潜力.
- 为了改造WS/DGAT酶以提高催化效率和基质耐受性.
- 探索合成乙烯基捐赠体模仿剂 (乙烯基-SNACs) 在酶性 Ester 合成中的使用.
主要方法:
- 使用了来自Streptomyces coelicolor的WS/DGAT酶.
- 采用合成的乙-SNACs作为乙-捐赠者的模仿.
- 进行结构建模以指导位点定向突变发生.
- 通过定向进化生成和表征一种变异酶 (L25F).
主要成果:
- 野生类型的WS/DGAT表现出与酒精受体和乙烯-SNACs的活性.
- 该酶对结构多样化的乙烯基捐赠体表现出广泛的基质耐受性.
- 与野生类型相比,L25F变体显示出明显提高的催化效率,特别是在芳香,短线和分支的乙烯基捐赠体中.
结论:
- 菌株Streptomyces coelicolor WS/DGAT是一种可行的生物催化剂,用于使用乙烯-SNACs生产 Ester.
- 通过结构建模和突变发生的酶工程可以提高所需 Ester 基质的催化效率.
- 该L25F变体代表了一个有前途的工具,用于工业合成有价值的短/分支 Ester.
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