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在中国福建省,从烧伤感染中分离的金黄色葡萄球菌分子的分子类型和特征

Xiaolan Hong^1,2, Shaobo Zhou², Xubo Dai²

¹Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fujian, China.

Frontiers in microbiology

|October 6, 2023

概括

这项研究确定了在中国福建导致烧伤感染的黄金葡萄球菌 (黄金葡萄球菌) 的流行分子类型. 一种具有强大的生物膜形成和毒性基因的新型温泉类型值得临床关注.

科学领域:

微生物学微生物学
流行病学流行病学
传染性疾病传染性疾病

背景情况:

黄金葡萄球菌 (S. aureus) 是烧伤感染的主要原因,导致显著的发病率和死亡率.
有限的数据存在于S. aureus的分子流行病学和抗菌素耐药性在中国福建的烧伤感染中.

研究的目的:

为了研究S. aureus从中国福建的烧伤感染中分离的分子特征,毒性因子,生物膜形成和抗菌敏感性.
为了在这个患者群体中识别S. aureus的流行克隆和新型菌株.

主要方法:

在2016年至2021年期间从烧伤中收集了90个S. aureus分离物 (59个MRSA,31个MSSA)
使用多位置序列类型 (MLST),spa类型,agr类型和SCCmec类型进行基因类型化.
通过PCR评估病毒性基因,通过TCP测定生物膜形成,并使用稀释方法评估抗菌敏感性.

主要成果:

确定了37种序列类型 (ST) 和34种spa类型,包括一种新型 (spa-t20720).
CC8-ST239-t030-agrI-SCCmecIII和CC7-ST7-t091-agrI分别是占主导地位的MRSA和MSSA克隆. 这两个类型的克隆分别占主导地位.
检测到12个毒性基因,其中icaA/icaD (100%) 是最常见的. 新的spa-t20720分离物表现出强烈的生物膜形成,并携带多个毒性基因.

结论:

CC8-ST239和CC7-ST7是福建烧伤感染中主要的MRSA和MSSA分子特征.
新型spa-t20720金黄色菌株具有广泛的毒性因子和强大的生物膜能力,需要提高临床警.

关键词:

黄金菌的毒性基因S. aureus的毒性基因对抗微生物的敏感性.生物膜形成的分离物.烧伤伤口感染感染分子类型的分子类型.

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