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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K
Light Acquisition02:16

Light Acquisition

8.5K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.5K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.2K

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相关实验视频

Updated: Jul 14, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

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自动机器人辅助广场光学连贯性断层扫描,使用结构光摄像头.

Xiaochen Li1, Yong Huang1, Qun Hao1

  • 1School of Optics and Photonics, Beijing Institute of Technology, No.5 South Zhongguancun Street, Haidian, Beijing, 100081, China.

Biomedical optics express
|October 6, 2023
PubMed
概括

本研究介绍了一种使用机器人手臂和结构光摄像头的自动化系统,以克服光学连贯断层扫描 (OCT) 中有限的视野. 该系统可为各种应用提供大面积的3D OCT成像.

科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 机器人技术 机器人技术 机器人技术
  • 光学工程是指光学工程.

背景情况:

  • 传统的光连贯断层扫描 (OCT) 系统具有有限的视野 (FOV),限制了它们的应用范围.
  • 需要先进的成像技术,能够进行高分辨率,大面积的3D重建.

研究的目的:

  • 开发和验证用于大面积OCT扫描的自动化系统.
  • 通过克服FOV限制,提高OCT在生物医学和材料检查中的适用性.

主要方法:

  • 整合了一个机器人手臂,3D结构光摄像头和一个便携式OCT探头.
  • 使用结构光摄像头生成3D点云,用于自动化扫描路径规划.
  • 将OCT探头安装在机器人手臂上,以便在预先确定的路径上精确地扫描样品.

主要成果:

  • 机器人手臂实现了准确的定位,平均绝对误差约为0.16毫米.
  • 该系统成功地生成了高分辨率,大面积的3D OCT重建.
  • 证明了脏,皮肤和叶片叶片样本的外体成像,面积超过20厘米.

结论:

  • 拟议的自动化系统有效地克服了海外国土和地区的FOV限制.

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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

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  • 该系统可实现准确高效的大面积3D OCT成像.
  • 这项技术具有生物医学,工业和农业应用的巨大潜力.