在日本一所大学医院对Clostridioides difficile分离物的分子流行病学分析
Yukitaka Ito1,2, Koichi Tanimoto3, Naoko Chiba2
1Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Tokyo, Japan.
Heliyon
|October 6, 2023
概括
日本Clostridioides difficile (C. diff) 的分子流行病学揭示了与医疗保健相关的常见菌株,ST8和ST17. 毒素基因表达与C.diff感染的发展没有显著的相关性.
科学领域:
- 分子流行病学分子流行病学
- 传染性疾病 传染性疾病
- 微生物学 微生物学
背景情况:
- 困难菌 (C. diff) 感染在医疗保健机构中构成重大风险.
- 了解C.diff的分子流行病学对于感染控制至关重要.
- 这项研究调查了日本一所大学医院的C. diff分离物.
研究的目的:
- 进行Clostridioides difficile分离物的分子流行病学分析.
- 为了揭示与日本一所大学医院C. difficile感染相关的危险因素.
- 为了研究C. diff毒素基因表达和感染发展之间的关系.
主要方法:
- 在2015年7月至2016年8月期间,从869名患者收集了919个便样本.
- 进行了毒素基因检测,核型,多位序列类型 (MLST) 和抗菌感应性测试.
- 使用定量实时聚合酶连锁反应 (qPCR) 进行C. diff毒素基因表达分析.
主要成果:
- 从153个样本 (16.6%) 中分离出了困难菌.
- 确定了在日本医疗保健相关感染中常见的ST8和ST17菌株.
- 在感染和非感染患者之间没有观察到C. diff毒素基因转录水平的显著差异.
结论:
- 主要的日本克隆菌株 (ST8,ST17) 在医院环境中持久,并导致与医疗保健相关的C. diff感染.
- C. diff毒素基因转录发生在感染和未感染的患者中.
- 毒素基因表达水平与C. diff感染的发展没有显著的关系.
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