基于Omics的数学建模揭示了实验性小鼠模型中牙周炎的病原性
C Fujihara1, K Murakami2, S Magi3
1Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
Journal of dental research
|October 6, 2023
概括
牙周炎的发病包括细菌和宿主因素. 数学建模揭示了细菌介导的Toll-like受体激活和单细胞招募是膜骨再吸收的关键驱动因素.
科学领域:
- 口腔生物学 口腔生物学
- 免疫学 免疫学 免疫学
- 系统生物学 系统生物学
背景情况:
- 牙周炎是一种复杂的多因素疾病,受细菌和宿主遗传因素的影响.
- 虽然omics分析已经确定了许多风险因素,但它们对牙周炎病原体的个别影响仍然不清楚.
研究的目的:
- 确定牙周炎病原发生的关键因素.
- 通过omics和数学建模,量化预测这些因素对膜骨再吸收的影响.
主要方法:
- 牙周炎在小鼠中通过牙绑定诱导.
- 评估了气膜骨再吸收,基因表达 (RNA测序) 和牙微生物组 (16S rRNA测序).
- 使用普通微分方程构建了一个数学模型来模拟疾病动态.
主要成果:
- 欧米克斯数据确定了牙周炎中关键的细菌和分子参与者.
- 以实验数据进行参数化的数学模型预测,通过细菌诱导的Toll-like受体激活来招募单细胞是膜骨再吸收的主要驱动因素.
- 骨质细胞介导的骨质细胞分化在牙周炎期间对骨完整性的影响较小.
结论:
- 细菌介导的托尔类受体激活和随后的单细胞招募是牙周炎中膜骨再吸收的主要调节者.
- 这项研究为了解牙周炎病原体和确定治疗点提供了定量框架.
相关概念视频
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...