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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 14, 2025

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

在人类生物流体中使用基于流量的分析仪评估细胞外囊泡.

Kevin Ho Wai Yim¹, Olga Krzyzaniak¹, Ala'a Al Hrout¹

¹Institute of Experimental Immunology, University of Zurich, Zurich, 8057, Switzerland.

Advanced healthcare materials

|October 6, 2023

概括

此摘要是机器生成的。

像NanoFCM这样的纳米分析仪提供卓越的细胞外囊泡 (EV) 分析,准确大小,量化和识别EV标记. 这种先进的流细胞计法揭示了具有临床潜力的生物流体中独特的EV签名.

关键词:

生物流体是一种生物流体.细胞外囊泡中的细胞外囊泡.液体活检是液体活检.纳米分析仪的使用方法纳米FCMM的使用方法

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Last Updated: Jul 14, 2025

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Characterizing Extracellular Vesicles from Biological Fluids

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

科学领域:

生物技术和生物医学工程生物技术和生物医学工程
分析化学分析化学
免疫学免疫学免疫学

背景情况:

细胞外囊泡 (EVs) 是重要的生物标志物,但由于它们的小尺寸和低折射率,分析是具有挑战性的,通常低于标准流动细胞计检测极限.
谱流分析仪和纳米分析仪代表了先进的细胞计技术,具有增强的灵敏度和分辨率用于粒子检测.
免疫细胞衍生的EV具有显著的临床潜力,但表征不佳,需要先进的分析技术来进行全面的表型.

研究的目的:

为了比较评估欧罗拉光谱流分析仪和NanoFCM纳米流分析仪的细胞外囊泡 (EV) 表型化性能.
评估这些先进的细胞计量平台对于分析来自免疫细胞的EV和来自人体生物流体的EV的适用性.
在生物流体中识别具有潜在临床意义的独特EV标记符号.

主要方法:

对Aurora (Cytek) 光谱细胞分析仪和NanoFCM (nFCM) 纳米流分析仪用于EV表型的比较分析.
使用B和T细胞系衍生的EV和人类生物流体衍生的EV (血,尿液,唾液),具有免疫标记物面板的特征.
评估了两个平台的EV尺寸准确性,颗粒度测量,标记子集识别和表面标记器共定位能力.

主要成果:

纳米FCM精确地分析小EV (40-200nm) 的尺寸精度与电子显微镜相比较.
纳米FCM精确地测量每体积单个EV粒子度,并识别不足的EV标记子集.
在人类生物流体中发现了独特的EV标记特征,这表明了未来的临床相关性;这两种平台对于不同的研究需求都有不同的优势.

结论:

纳米FCM提供细胞外囊泡 (EVs) 的高分辨率分析,在尺寸准确度,量化和详细标记分析方面表现出色.
这项研究强调了纳米分析器在特征免疫细胞衍生的EV和在生物流体中发现临床相关的EV特征方面的潜力.
光谱和纳米流细胞计平台都提供了独特的优势,满足了EV分析中的各种研究兴趣.