作为辅助剂的拉尼蒂丁调节巨细胞极化,并通过PI3K-Akt2信号通路激活CTLs
Chenglin Li1, Shuang Wang2, Xiaoran Ma2
1Department of Human Anatomy, Histology and Embryology, School of Basic Medicine, Qingdao University, Qingdao 266071, Shandong, China.
International immunopharmacology
|October 6, 2023
概括
拉尼蒂丁 (RAN) 激活巨细胞并增强免疫反应,显示出作为癌症疫苗新型辅助剂的潜力. 这项研究证明了RAN.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 药理学 药理学是指药理学的学科.
背景情况:
- 辅助剂对于疫苗的有效性至关重要,但人类疫苗的选择有限.
- 众所周知,像ranitidine (RAN) 这样的H2受体抑制剂具有增强免疫力的特性.
- 兰尼蒂丁的特定免疫增强作用和辅助潜力在很大程度上仍未被探索.
研究的目的:
- 为了研究兰尼蒂丁 (RAN) 作为一种增强免疫力的辅助剂的潜力.
- 阐明RAN影响巨细胞极化和免疫细胞激活的机制.
- 在小鼠瘤模型中评估RAN作为辅助剂的疗效.
主要方法:
- 在体外分析RAW264.7细胞,使用流细胞计,西部涂抹和免疫光.
- 研究信号通路,包括NF-κB和PI3K-Akt2.
- 使用B16-OVA瘤模型在OVA加RAN疫苗接种小鼠中的体内评估.
主要成果:
- 拉尼提丁治疗通过NF-κB和PI3K-Akt2通路促进了M1巨细胞的两极分化.
- 通过Akt2/GSK3β/Dynmin1信号传递,RAN增强了巨细胞内细胞功能.
- 在体内,RAN辅助疗法显著上调M1巨细胞,Th1细胞和CTLs,抑制瘤生长并改善生存率.
结论:
- 拉尼蒂丁有效地激活免疫系统,特别是促进M1巨分化和适应性免疫.
- 作为癌症疫苗的新型辅助剂,RAN显示出显著的潜力.
- 这项研究为RAN的免疫激活及其在瘤免疫治疗中的应用提供了机理性的见解.
更多相关视频
10:35Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
Published on: December 26, 2017
8.3K
11:48Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Published on: May 31, 2018
11.5K
相关概念视频
01:28Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists
495
Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
495
01:28Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists
176
Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy. SP binds and activates...
176
01:09GPCRs Regulate Adenylyl Cylase Activity
5.6K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.6K