代谢性压力对BeWo同胞细胞功能的影响
Lisa M Rogers1, Marissa Huggins2, Ryan S Doster3
1Division of Infectious Diseases, Department of Medicine, Indiana University School of Medicine, 545 Barnhill Dr., EH 305, Indianapolis, IN, 46202, USA.
Chembiochem : a European journal of chemical biology
|October 6, 2023
概括
母亲的代谢压力,模仿糖尿病和肥胖症,损害了同胞性细胞 (STB) 的功能. 这项体外研究表明,在暴露于代谢应激尾酒的STB类细胞中,激素和矩阵金属蛋白酶 (MMP) 生产发生变化.
科学领域:
- 生殖生物学和胎盘发育
- 细胞和分子内分泌学细胞和分子内分泌学.
- 代谢性疾病研究研究.
背景情况:
- 同胞性细胞 (STBs) 对于胎盘功能至关重要,调解交换并形成屏障.
- 热囊细胞功能,包括矩阵金属蛋白酶 (MMP) 分泌,对于胎盘发育至关重要.
- 肥胖和糖尿病等母亲的代谢状况会对胎儿发育和 trofhoblast 功能产生负面影响.
研究的目的:
- 为了建模代谢压力对STB功能的影响.
- 为了研究在代谢应激条件下MMP和激素分泌的变化.
主要方法:
- 利用BeWo细胞细胞系,使用福斯科林诱导形成STB类细胞.
- 通过电子显微镜和免疫光学评估细胞形态.
- 在暴露于代谢应激尾酒 (MetaC) 后,使用ELISA和RT-qPCR量化表型.
主要成果:
- 通过增加HCGβ和孕激素分泌和高丁基因表达的成功合成证实了成功的合成.
- 代谢性压力 (MetaC) 显著抑制了合成丁基因表达 (ERVW-1,ERVFRD-1).
- MetaC降低了HCGβ和孕激素分泌,并改变了MMP-2和MMP-9的产生.
结论:
- 在体外代谢压力,模拟糖尿病和肥胖症,对STB激素和MMP生产产生不利影响.
- 这些发现突出了母亲代谢障碍影响胎盘功能的潜在机制.
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