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玻璃化对胚胎细胞线粒体DNA动态和基因表达特征的影响

Marta Pérez-Sánchez¹, Maria Luisa Pardiñas¹, Antonio Díez-Juan²

¹IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106 - Torre A, Planta 1ª, 46026, Valencia, Spain.

Journal of assisted reproduction and genetics

|October 6, 2023

概括

玻璃化和变暖暂时降低了人体胚芽细胞中的线粒体DNA (mtDNA) 含量,但水平在几个小时内恢复,以及与发育和细胞应激相关的基因表达变化.

科学领域:

生殖生物学生殖生物学
胚胎学胚胎学
遗传学是一个遗传学.

背景情况:

玻璃化是一种常见的方法,用于保存人类胚胎囊.
玻璃化和变暖对线粒体DNA (mtDNA) 含量和胚芽细胞基因表达的影响需要进一步调查.

研究的目的:

为了研究玻璃化/加热对线粒体DNA (mtDNA) 含量和人类胚胎细胞的基因表达特征的影响.

主要方法:

一项前性队列研究,涉及50名患者的89个芽细胞囊.
使用实时聚合酶链反应 (RT-PCR) 测量线粒体DNA (mtDNA) 含量.
热后0小时和4-5小时通过RNA测序 (RNA-seq) 进行的转录组分析.

主要成果:

在加热后的第一个小时内观察到mtDNA含量显著下降.
热后4-5小时内mtDNA含量显著增加,恢复到玻璃化前的水平.
基因表达分析揭示了与胚胎发育和细胞应激反应相关的途径的上调,包括亡和DNA损伤.

结论:

这是第一个证明人类胚胎细胞mtDNA含量因玻璃化和变暖而受到调节的研究.
这些发现提供了对冷保存后人体胚胎囊中激活的途径的见解,有助于了解温暖后的胚胎生存能力.

关键词:

胚胎应激反应的压力反应线粒体中的线粒体.在RNA-seqqq.玻璃化-加热-加热 mtDNA 含量 mtDNA 的含量

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