使用疏水性树脂排毒松树林垃圾水解剂和综合发酵工艺开发补充糖蜜的增强乙醇生产
Ajay Kumar Pandey1,2, Sangeeta Negi3
1Department of Life Sciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, Kanpur, 208024, Uttar Pradesh, India. ajaynitbiotech@gmail.com.
Environmental science and pollution research international
|October 6, 2023
概括
松树林垃圾 (PFL) 的优化预处理和随后的发酵,特别是用糖蜜补充,显著提高了生物乙醇的生产. 这种混合方法为可持续燃料开发提供了一个可行的战略.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 生物化学工程 生物化学工程
背景情况:
- 全球不断增长的乙醇需求需要先进的生物燃料生产方法.
- 整合第一代和第二代原料是实现可持续生物乙醇目标的关键.
- 松树林垃圾 (PFL) 是一个未充分利用的纤维素化合物资源.
研究的目的:
- 优化松树林垃圾 (PFL) 的两步预处理过程.
- 为了提高PFL水解盐的糖化和发酵,用于生产生物乙醇.
- 为了评估糖蜜补充和抑制剂去除工业规模应用的疗效.
主要方法:
- 开发了一种优化的两步序列预处理 (然后酸) 的PFL.
- 采用响应表面方法 (RSM) 采用盒式设计,以优化糖化.
- 使用Saccharomyces cerevisiae进行发酵,并通过XAD-4树脂处理评估了通过XAD-4树脂处理去除抑制剂.
主要成果:
- 在优化糖化条件下达到0.558g/g的降低糖释放量.
- 从PFL水解剂产生22.51g/L的乙醇,在去除抑制剂后增加到27.38g/L.
- 使用糖蜜补充剂达到46.02g/L乙醇,产量高 (0.482g/g) 和生产率高 (1.92g/L/h).
结论:
- 优化的PFL预处理和糖化对于生物乙醇生产是有效的.
- 疏水性树脂处理成功降低了抑制剂,提高了乙醇产量.
- 用糖蜜补充红纤维素解盐为工业生物乙醇生产提供了一个有前途的混合技术.
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