STAT3依赖的长非编码RNALncenc1通过稳定Klf4mRNA,促进小鼠ES细胞的多能性
Emanuele Monteleone1,2, Paola Corrieri1, Paolo Provero1
1Department of Molecular Biotechnology and Health Science, University of Torino, Via Nizza 52, 10126 Torino, Italy.
Briefings in functional genomics
|October 6, 2023
概括
白血病抑制因子 (LIF) 激活的STAT3维持胚胎干细胞 (ESC) 的干性. 一个新的长非编码RNA,Lncenc1,与STAT3在积极的反循环中起作用,通过调节Klf4表达来支持ESC多能性.
科学领域:
- 干细胞生物学 干细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 非编码RNA的研究.
背景情况:
- 胚胎干细胞 (ESC) 具有自我更新和分化潜力.
- 白血病抑制因子 (LIF) 激活的STAT3信号对于维持ESC干性至关重要.
- 在ESC中调节非编码RNA中的STAT3的作用仍然在很大程度上未被探索.
研究的目的:
- 调查STAT3是否直接调节干细胞特异性的非编码RNA.
- 为了确定涉及LIF-STAT3介导的ESC多能性的新型非编码RNA.
- 阐明已识别的非编码RNAs对干性有所贡献的机制.
主要方法:
- 生物信息管道在小鼠ESC中的应用.
- 识别和表征STAT3依赖的长非编码RNAs.
- 分析Lncenc1在细胞质中的作用及其与microRNA-128和Klf4mRNA的相互作用.
主要成果:
- 识别Lncenc1作为STAT3依赖的长非编码RNA,支持小鼠ESC多能性.
- 在细胞质中,lncenc1的功能是积极调节LIF-STAT3轴.
- Lncenc1通过与microRNA-128竞争与Klf4 3'UTR结合来增强Klf4的表达.
结论:
- 已经发现了一种基于LIF-STAT3介导的多能性的一种新的非编码RNA机制.
- Lncenc1作为STAT3通路的正反调节器,对维持ESC干性至关重要.
- 这一发现扩大了我们对管理干细胞命运的调节网络的理解.
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