转录因子NAC1激活了根中的酶编码AtCEPs的表达,以限制根毛发的生长
Diana R Rodríguez-García1, Yossmayer Del Carmen Rondón Guerrero1, Lucía Ferrero2
1Fundación Instituto Leloir and IIBBA-CONICET. Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentina.
Plant physiology
|October 6, 2023
概括
植物囊内酶 (CEP) 通过处理扩展酶来调节根毛发 (RH) 的生长. NAC1转录因子控制CEP,影响RH延长和Arabidopsis细胞壁的形成.
科学领域:
- 植物分子生物学 植物分子生物学
- 细胞壁生物学 细胞壁生物学
- 植物开发 植物开发
背景情况:
- 植物基因组用KDEL ER保留信号 (CEP) 编码帕帕因类型的囊内酶 (CysEPs).
- 在CEP过程中,Extensins (EXTs) 对于细胞壁的形成和扩张至关重要.
- 通过削弱细胞壁,CEP可以调节细胞延长.
研究的目的:
- 研究阿拉比多普西斯CEPs (AtCEPs) 在根毛发 (RH) 生长中的作用.
- 确定控制RHs中的AtCEP表达的调节机制.
- 阐明在RH延长过程中细胞壁修饰中AtCEPs的功能.
主要方法:
- 对AtCEPs (atcep1-3,atcep2-2,atcep3-2) 的Arabidopsis单个和双重突变的分析.
- 对NAC1转录因子进行基因表达分析 (RT-qPCR) 和染色体免疫沉 (ChIP).
- 对NAC1.1的过度表达和主要抑制剂研究.
- 在RH细胞壁中分析延长素沉积.
主要成果:
- 在RH细胞文件中,AtCEP基因的表达高.
- 亚特塞普双突变体呈现较长的RH,表明AtCEPs抑制RH延长.
- 转录因子NAC1激活AtCEP的表达,并限制RH的生长.
- NAC1直接与AtCEP促进体结合,调节它们的表达.
- 在atcep突变体中的RH细胞壁显示了减少的Extensin沉积.
结论:
- AtCEPs在抑制极地根毛发生长方面发挥着至关重要的作用.
- NAC1作为AtCEP表达的积极调节者,控制RH延长.
- AtCEPs通过处理和不溶性化Extensins来改变细胞壁的功能.
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