使用离子对液态色谱/并联质谱法在人体血中确定未衍生氨基酸
Cheryl Turner1, Helga Refsum2, Nasser E Bastani3
1Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
概括
本研究提出了一种经过验证的液体染色学-并联质谱法方法,用于准确量化人体血中的氨基酸. 该试验表现出高准确度,精度和灵敏度,使其适合临床和研究应用.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 准确的氨基酸量化对于临床研究,食品分析和制药研究至关重要.
- 现有的方法可能需要更大的样本量或缺乏对关键氨基酸的全面分析.
研究的目的:
- 开发和验证一种敏感的液体染色学-并联质谱法 (LC-ESI-MS/MS) 方法,用于量化人体血中的多种氨基酸.
- 为了建立一个强大的测定牛,肌素,谷氨 (GSH) 和含硫氨基酸 (SAA).
主要方法:
- 使用液体色谱与喷射电离子双重质谱 (LC-ESI-MS/MS) 进行氨基酸分析.
- 在C18柱上使用七黄油酸 (HFBA) 作为离子配对剂,以实现最佳分离.
- 纳入稳定同位素标记的氨基酸内部标准 (IS) 和多重反应监测 (MRM) 进行精确的量化.
主要成果:
- 该方法在一系列氨基酸中表现出极好的线性 (r > 0.9927) 和准确性 (92-110%).
- 获得了高精度 (<15%) 和敏感检测极限 (LOD:0.001-1.80μM,LOQ:0.004-6.0μM).
- 没有观察到显著的离子抑制或转移,证实了测试的稳定性.
结论:
- 开发的LC-ESI-MS/MS测定被验证为准确,精确和灵敏地量化人体血中的关键氨基酸.
- 该方法的可靠性和效率使其适用于各种分析应用,包括临床诊断和研究.
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