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负DNA超引发全基因组的Cas9目标外活动

Matthew D Newton1, Marialucrezia Losito2, Quentin M Smith3

  • 1Department of Infectious Disease, Faculty of Medicine, Imperial College London, Du Cane Road, London W12 0HS, UK; Single Molecule Imaging, MRC-London Institute of Medical Sciences, Du Cane Road, London W12 0HS, UK; DSB Repair Metabolism Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Molecular cell
|October 6, 2023
PubMed
概括

像负超卷一样,DNA拓学显著增加了CRISPR-Cas9基因编辑的目标外活动. 这一发现揭示了细胞过程如何无意中产生意外的DNA断裂,影响治疗安全.

关键词:
这就是CRISPR/Cas9的作用.细胞编辑 细胞编辑在DNA拓学上.基因编辑 基因编辑全基因组范围的目标之外的目标.负超卷是指负超卷.下一代测序是什么意思光学子 - 光学子单分子显微镜的显微镜.转录合的目标之外的目标.

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