使用FRET光谱学描述PEG涂层ZnO纳米晶体的联结体形态
Danielle R Lustig1, Enes Buz2, Justin T Mulvey3,4
1Department of Chemistry, Colorado State University, 200 West Lake Street, Fort Collins, Colorado 80523-1872, United States.
The journal of physical chemistry. B
|October 6, 2023
概括
在ZnO纳米晶体 (NCs) 上分析了聚乙烯甘醇 (PEG) 连接体,使用弗斯特共振能量转移 (FRET) 光谱学. 结果显示PEG的外是多孔或不齐的,而不是刚性的障碍物,影响药物载体的透性.
科学领域:
- 纳米技术纳米技术
- 材料科学 材料科学 材料科学
- 生物物理学的生物物理.
背景情况:
- 聚乙烯甘醇 (PEG) 连接物对基于纳米晶体 (NC) 的药物载体的表面被动化至关重要,防止生物分子粘附.
- 了解PEG连接体外形态对于控制药物载体的透性和有效性至关重要.
- 现有的分析工具很难在现场描述PEG外结构,从而限制了对其屏障特性的了解.
研究的目的:
- 开发和应用一种基于弗斯特共振能量转移 (FRET) 光谱学的方法,通过PEG涂层的ZnONCs来评估分子透性.
- 在ZnONC上研究PEG连接体外的现场形态,作为连接体链长度的函数.
- 为了确定PEG连接物是否在NC表面形成不可穿透的屏障或多孔结构.
主要方法:
- 用变量链长的PEG-silane连接物涂层的ZnONCs的合成.
- 使用FRET光谱学,以ZnONCs作为捐赠物和扩散分子作为接受物.
- 使用时间解析光发光寿命 (TRPL) 谱学的量化能量转移 (EnT) 效率.
主要成果:
- 能量传输效率被测量为 PEG 带链长度的函数.
- 意想不到的是,最长的PEG-西兰联体表现出与裸体ZnONCs相似的EnT效率.
- 这表明PEG外不是一个刚性,难以穿透的屏障,而是很可能是多孔的或处于"状态".
结论:
- 对于NC上的PEG连接体的"刚性外"模型不足以解释观察到的透性.
- 开发的FRET光谱学方法提供了在生物条件下对联体透性有价值的现场信息.
- 研究结果表明,PEG外结构更具动态性和透性,影响了基于NC的药物递送系统的设计.
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