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肥胖症 改变细胞因子信号传递和败血症小鼠肠道微生物组
Lauren Bodilly1, Lauren Williamson2, Patrick Lahni2
1Department of Pediatrics, University of Iowa, Iowa City, Iowa, USA.
Innate immunity
|October 6, 2023
概括
肥胖通过改变细胞因子配置和肠道微生物组,改善了败血症小鼠的生存率. 在肥胖小鼠中,较低的瘤亡因子-α (TNFα) 和互白素-6 (IL-6) 水平与更好的结果相关.
科学领域:
- 免疫学 免疫学 免疫学
- 代谢障碍 代谢障碍 代谢障碍
- 微生物组研究 微生物组研究
背景情况:
- 败血症是一个关键的全球健康问题,死亡率高.
- 血细胞因子水平是败血症预后的潜在指标.
- 肥胖对败血症诱导的细胞因子变化和存活率的影响尚不清楚.
研究的目的:
- 为了研究肥胖如何影响早期败血症期间的细胞因子概况.
- 为了确定改变的细胞因子水平和肥胖与非肥胖的败血症小鼠的生存之间的相关性.
- 探索肠道微生物在与肥胖相关的败血症结果中的作用.
主要方法:
- C57BL/6雄性小鼠被食控制或高脂肪饮食以诱导肥胖.
- 败血症在实验中使用结和穿孔 (CLP) 来诱导.
- 在CLP8小时后测量了血细胞因子水平 (adiponectin,leptin,resistin,TNFα,IL-6);监测了存活率.
主要成果:
- 肥胖小鼠在败血症诱导后表现出显著更高的生存率.
- 败血性肥胖小鼠显示阿迪波内克丁,莱普和抵抗素水平升高,但在8小时后降低了TNFα和IL-6水平.
- 在幸存下来的肥胖小鼠中观察到较低的TNFα和IL-6,而在24小时内死亡的非肥胖小鼠中发现了更高的水平.
- 饮食和败血症都显著改变了脑膜微生物组合.
结论:
- 在小鼠败血症模型中,肥胖与改善的生存率有关.
- 介乐-6 (IL-6) 作为肥胖和非肥胖个体早期败血症的预后生物标志物.
- 肠道微生物组及其对败血症的反应的差异可能是肥胖小鼠观察到的生存优势的基础.
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