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扩展显微镜的trichomonads 的扩展显微镜

Paula Terra Bandeira¹, Sharmila Fiama das Neves Ortiz², Marlene Benchimol³

¹Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Pesquisa em Medicina de Precisão, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-901, Brazil; Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens e Centro Nacional de Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-901, Brazil.

Experimental parasitology

|October 6, 2023

概括

扩展显微镜 (ExM) 提高了寄生虫成像分辨率. 这种技术可以改善细胞骨蛋白质的可视化,例如Trichomonas vaginalis和Tritrichomonas foetus中的管蛋白.

科学领域:

细胞生物学细胞生物学
显微镜技术显微镜技术
寄生虫学的寄生虫学

背景情况:

光显微镜的最新进展集中在提高光成像的分辨率上.
扩展显微镜 (ExM) 是一种物理扩展生物样本以增强空间分辨率的技术.

研究的目的:

将扩展显微镜 (ExM) 应用到寄生虫Trichomonas vaginalis和Tritrichomonas胎儿身上.
用免疫光显微镜改善细胞骨相关蛋白质的局部化和可视化.

主要方法:

对Trichomonas vaginalis和Tritrichomonas胎儿的样本被嵌入到一个水友,可膨胀的凝中.
凝被同位素扩展了4.5倍,达到大约70nm的空间分辨率.
免疫光显微镜被用来可视化特定的蛋白质,包括素和 costain 1.

主要成果:

在这两种寄生虫中,ExM显著提高了免疫光成像的分辨率.
对原生动物的结构组织进行了更详细的观察.
用光显微镜首次可视化了轴突的个别微管.
实现了管和 costain 1 的详细定位,揭示了对 costa 结构的新见解.

结论:

ExM是对原生虫寄生虫的高分辨率成像的一个有价值的工具.
该技术增强了对这些生物体中细胞骨组织和蛋白质定位的研究.
ExM为了解寄生虫超结构和分子组件提供了前所未有的细节.

关键词:

在Axostyle中使用.科斯塔科斯塔科斯塔科斯塔科斯塔科斯塔细胞骨架细胞骨架阴道的三虫 (Trichomonas vaginalis) 已经被发现.三基马病毒胎儿

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