相关实验视频

Updated: Jul 14, 2025

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

一个PrPEGFR信号轴通过调节细胞能量通路来控制神经干细胞衰老

Bradley R Groveman¹, Benjamin Schwarz², Eric Bohrnsen²

¹Laboratory of Neurological Infections and Immunity, National Institute of Allergy and Infectious Diseases, Division of Intramural Research, Rocky Mountain Laboratories, National Institutes of Health, Hamilton, Montana, USA.

The Journal of biological chemistry

|October 6, 2023

概括

蛋白 (PrP) 缺失通过降低表皮生长因子受体 (EGFR) 信号和线粒体功能来损害神经干细胞 (NSC) 的生长,导致增殖减少和衰老增加.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

尽管它与神经退行性疾病有关,但对蛋白 (PrP) 的原生功能知之甚少.
PrP影响细胞增殖,衰老和表皮生长因子受体 (EGFR) 信号传递.
成人神经干细胞 (NSC) 对大脑可塑性至关重要,但它们通过PrP的调节尚不清楚.

研究的目的:

研究PrP在调节细胞衰老,EGFR信号传递和成年小鼠神经干细胞 (NSC) 中线粒体功能中的作用.

主要方法:

在实验室中对PrP淘汰赛 (KO) 和野生型 (WT) 的NSC进行比较.
分析EGFR信号传递,细胞衰老标志物和线粒体功能.
细胞能量通路的RNA测序和代谢分析.

主要成果:

与WT NSC相比,PrP KO NSCs表现出减少的增殖和增加的衰老.
在PrP KO NSC中,EGFR表达减少,而EGF补充剂减轻了衰老.
RNA-seq和代谢分析揭示了PrP KO NSC中EGFR信号的下调,线粒体功能的降低和能量代谢受损.

结论:

蛋白 (PrP) 对于维持神经干细胞 (NSC) 增殖和功能至关重要.
缺少PrP会导致EGFR信号受损,线粒体能量产生减少,细胞衰老.
这些发现表明,PrP在通过能量代谢和信号传递来调节NSC生长途径方面发挥着关键作用.

关键词:

欧洲农业基金会 (EGFR) 是一个基金.这就是为什么PrPP是PrP.皮肤表皮生长因子葡萄糖溶解是什么线粒体中的线粒体.普里昂是什么意思?普里昂是什么意思?衰老是一种老化.

更多相关视频

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Induction and Validation of Cellular Senescence in Primary Human Cells

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

相关概念视频

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...