PsDMAP1蛋白在调节Phytophthora sojae的生长和病原性方面的新功能
Fan Zhang1, Shanshan Chen1, Tongshan Cui1
1Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
International journal of biological macromolecules
|October 6, 2023
概括
基因甲基转移酶1相关蛋白 (DMAP1) 对菌体P. sojae至关重要. 沉默PsDMAP1会影响生长,繁殖和毒性,并且它与DNA甲基转移酶相互作用以调节基因表达.
科学领域:
- 分子生物学分子生物学
- 植物病理学 植物病理学
- 遗传学 是一个遗传学.
背景情况:
- 已知DNA甲基转移酶1-关联蛋白 (DMAP1) 与DNA甲基转移酶1 (DNMT1) 相互作用,并参与基因素乙化和染色体重塑.
- 在线索性病原体中DMAP1的特定功能和调节机制在很大程度上仍未被探索.
- 虫病原体对农业构成重大威胁,需要对它们的分子机制有更深入的了解.
研究的目的:
- 为了识别和表征大豆病原体P. sojae (大豆病原体) 中的DMAP1基因.
- 研究PsDMAP1在P. sojae发育中的作用,应激反应和致病性.
- 探索PsDMAP1与DNA甲基转移酶的相互作用及其在表观遗传调节中的作用.
主要方法:
- 生物信息分析以识别PsDMAP1的正义.
- 在真核生物中对DMAP1序列的遗传学分析.
- 在P. sojae中进行基因淘汰和沉默实验.
- 对生长,繁殖和毒性进行表型分析.
- 压力测试用于反应性氧物种 (ROS) 和透性耐受性.
- 同免疫沉和酶活性试验用于研究蛋白质相互作用.
主要成果:
- PsDMAP1被确定为人类DMAP1的P. sojae正义体,具有保留的基因素尾部结合域.
- 对PsDMAP1的同卵性淘汰导致了P. sojae的死亡率.
- 沉默PsDMAP1显著降低了菌根的生长,体和动物体的产生,囊体的发芽和毒性.
- PsDMAP1对于P. sojae对ROS和透应激的反应至关重要.
- PsDMAP1与DNAN6-甲基亚丁胺甲基转移酶PsDAMT1相互作用,增强其活性并调节6mA水平.
结论:
- 在P. sojae的生长,发育,应激反应和病原性方面,PsDMAP1起着至关重要的作用.
- PsDMAP1 作为oomycetes中DNA N6-甲基亚丁 (6mA) 甲基化的一种新型调节剂.
- 这项研究提供了关于DMAP1在植物病原性菌体中的功能的首次见解,突出了其作为疾病控制目标的潜力.
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