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在结性脊髓炎中DNA甲基化和LGR6基因的表达:一个病例对照研究

Yujie Deng¹, Wei Xu¹, Man Ni¹

¹Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China; The Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.

Human immunology

|October 6, 2023

概括

氨酸丰富的重复含有G蛋白合受体6 (LGR6) 的DNA低甲基化和减少的转录水平与结性脊髓炎 (AS) 有关. 这种表观遗传失调可能在AS的发病过程中发挥作用.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

脊髓炎 (AS) 是一种慢性炎症性疾病,主要影响脊椎.
人们越来越认识到表观遗传修饰的作用,例如DNA甲基化,在AS病原体中发挥作用.
氨酸丰富的重复含G蛋白合受体6 (LGR6) 是一种基因,其在AS中的功能尚不清楚.

研究的目的:

研究氨酸丰富的重复含有G蛋白结合受体6 (LGR6) 的DNA甲基化和结性脊髓炎 (AS) 中的转录水平之间的关系.
探索LGR6甲基化作为AS诊断生物标志物的潜力.
阐明导致AS发展的表观遗传机制.

主要方法:

针对性双硫酸盐测序用于分析81例AS病例和81例对照的LGR6DNA甲基化.
定量实时转录-聚合酶链反应 (qRT-PCR) 测量了70例AS病例和64例对照的外周血液单核细胞 (PBMC) 中的LGR6转录水平.
接收器操作特征 (ROC) 曲线分析评估了LGR6甲基化的诊断性能.

主要成果:

与对照组相比,在两个CpG岛屿 (LGR6_1和LGR6_2) 中观察到LGR6的显著低甲基化在AS患者中.
在AS患者中,LGR6转录水平显著降低.
在LGR6 DNA甲基化水平和LGR6转录水平之间发现了正相关性,这表明了调节作用.
LGR6甲基化显示了AS的良好诊断性能 (AUC = 0.676).
性别可能会影响LGR6_1甲基化模式.

结论:

对LGR6DNA甲基化的表观遗传失调与结性脊髓炎的发病有关.
LGR6甲基化作为AS的潜在表观遗传生物标志物.
这些发现为识别生物标志物和开发AS治疗策略提供了新的途径.

关键词:

结性脊柱炎是一种疾病.通过DNA甲基化.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.在LGR6中,LGR6是LGR6.

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