患有初发性和复发性病的儿童的肠道微生物组:试点研究和探索性分析
Jonathan S Ellison1, Samantha N Atkinson2, Mike Hayward2
1Division of Pediatric Urology & Department of Urology, Children's Wisconsin & Medical College of Wisconsin, Milwaukee WI, USA.
Journal of pediatric urology
|October 6, 2023
概括
儿科结石疾病正在上升,在儿童中具有明显的生物机制. 这项试点研究发现,小儿石复发患者的微生物多样性和氧酸盐基因表达较低,这表明潜在的诊断标志物.
科学领域:
- 儿科脏病学 儿科脏病学
- 微生物组研究 微生物组研究
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 在儿童中,结石疾病 (结石) 的发病率正在增加.
- 显而易见的生物机制区分儿科和成人结石疾病.
- 影响重复儿科结石的因素及其微生物组关联性尚不清楚.
研究的目的:
- 为了比较儿童的微生物组信号,初始与复发性结石病.
- 探索小儿结石形成者的微生物组合,多样性和尿路参数之间的关联.
- 调查儿童中石复发事件的潜在诊断标志物.
主要方法:
- 试点队列研究16名儿童 (年龄在6-18岁) 具有结石病史.
- 使用16S核糖体DNA测序对微生物群多样性的便样本的分析.
- 枪支测序以评估氧化酶降解和丁酸盐生产基因,与石头复发和尿路参数相关.
主要成果:
- 经常出现的儿科结石形成者表现出较低微生物多样性的趋势 (Faith和Shannon指数).
- 在初始结石者中,与反复结石者相比,观察到Formyl-CoA转移酶的平均丰度更高的趋势.
- 在患有高性尿症的儿童中,Eubacterium siraeum的数量显著增加 (p=0.001).
结论:
- 试点研究结果表明,在儿科重复性结石患者中,微生物多样性较低,氧酸盐基因表达发生变化.
- 这些微生物组的变化可能作为未来结石事件的潜在诊断标记.
- 需要进一步的研究,以在更大的队列中验证这些发现.
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