YTHDF2以一种独立的方式通过UPF1促进了侵略性形成
Hyun Jung Hwang1, Tae Lim Park1, Hyeong-In Kim1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.
Nature communications
|October 6, 2023
概括
YTHDF2蛋白在细胞蛋白质质量控制中发挥着至关重要的作用,它有助于通过独立于其已知的RNA结合功能而形成攻击性细胞. 它的缺失会损害错误折叠的蛋白质的运动,促进细胞死亡.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- YTHDF2被称为一种RNA结合蛋白,其向N6甲基氨酸 (m6A) 修饰的RNA进行降解.
- 蛋白质稳态依赖于无素-蛋白酶体系统和侵略体的形成来隔离错误折叠的蛋白质.
研究的目的:
- 调查YTHDF2在侵略性形成中的作用,独立于其m6ARNA结合活性.
- 阐明YTHDF2影响错折聚的运动的机制.
主要方法:
- 在低调节YTHDF2表达的HeLa细胞中研究了YTHDF2功能.
- 分析了攻击体的形成,蛋白质聚合物的运动和细胞亡.
- 研究了涉及YTHDF2,UPF1和dynein的蛋白质-蛋白质相互作用.
主要成果:
- 降低YTHDF2的调节损害了攻击性细胞的形成,并降低了错误折叠的多的运动速度.
- YTHDF2与UPF1相互作用,形成一个复杂的错误折叠的多.
- YTHDF2增强了dynein运动蛋白的相互作用,促进了多的运输到侵略体.
- 降低YTHDF2水平促进了细胞亡.
结论:
- YTHDF2在蛋白质质量控制中具有关键的独立功能.
- YTHDF2通过与dynein运动复合体的相互作用,促进错误折叠的蛋白质的运输到侵略体.
- YTHDF2是侵略性细胞形成和细胞对蛋白质错折的反应的关键调节者.
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