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相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Updated: Jul 14, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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下一代测序用于研究DNA相互作用.

Nachammai Kathiresan1, Srinithi Ramachandran2, Langeswaran Kulanthaivel1,3

  • 1Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.

Methods in molecular biology (Clifton, N.J.)
|October 6, 2023
PubMed
概括

下一代测序 (NGS) 彻底改变了基因组学,使得DNA和RNA的快速分析成为可能. 这项技术推动了疾病,个性化医学和环境科学方面的发现.

关键词:
癌症 癌症 癌症 癌症互动 DNA 互动 DNA 互动传染病 传染病 传染病 传染病这是下一代测序.测序测序是指测序的时间.

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 下一代测序 (NGS) 提供了高速,准确和具有成本效益的DNA和RNA测序.
  • 在过去的十年中,NGS平台如Illumina,PacBio和Oxford Nanopore的重大进展已经发生.
  • 对于研究DNA相互作用和生物过程而言,NGS技术至关重要.

研究的目的:

  • 突出NGS对基因组学研究的变革性影响.
  • 要强调NGS在识别遗传变异和与疾病相关的修改中的作用.
  • 强调NGS在个性化医学和向治疗中的应用.

主要方法:

  • 使用各种NGS平台 (Illumina,PacBio,牛津纳米孔) 进行DNA和RNA测序.
  • 分析测序数据以识别遗传变异,基因表达模式和表观遗传修饰.
  • 应用NGS用于发现致病突变和生物过程调节.

主要成果:

  • NGS促进了与癌症,神经系统疾病和传染病有关的遗传变异的发现.
  • 通过NGS分析识别关键的生物过程和细胞信号通路.
  • NGS使得开发有针对性的药物和个性化治疗策略成为可能.

结论:

  • 下一代测序从根本上改变了基因组学领域.
  • NGS是了解DNA相互作用的强大工具,在医学和环境科学中具有广泛的应用.
  • 随着NGS的不断发展,它有望在生物和医学研究领域取得进一步的突破.