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铁复合体和安培素对黄金葡萄球菌 (Staphylococcus aureus) 的协同抗菌作用

Ludmila Kosaristanova¹, Martin Rihacek¹, Frantiska Sucha¹

¹Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic.

BMC microbiology

|October 6, 2023

概括

这项研究表明,将铁复合物 (Fe16) 与安培 (AMP) 结合起来,可以有效地杀死金黄色葡萄球菌. 这种组合疗法提供了一种有前途的新策略来对抗抗生素耐药细菌.

科学领域:

微生物学微生物学
材料科学材料科学材料科学
生物化学生物化学

背景情况:

像金黄色葡萄球菌 (Staphylococcus aureus) 这样的细菌的抗生素耐药性是全球卫生危机.
金属复合物正在被探索作为潜在的抗菌剂或现有抗生素的辅助剂.
金属复合物和抗生素之间的协同作用可能会增强细菌对抗菌治疗的敏感性.

研究的目的:

合成和表征一种铁复合物 (Fe16).
评估Fe16与安培 (AMP) 结合对抗S. aureus的协同抗菌活性.
调查Fe16+AMP组合的作用机制和安全概况.

主要方法:

铁复合物 (Fe16) 的合成和光谱表征.
查克板测试以确定分数抑制度指数 (FICI) 和最小抑制度 (MIC).
评价膜破坏,排泄的基因表达,以及对角质细胞的细胞毒性.

主要成果:

与单独使用AMP或Fe16相比,Fe16+AMP组合的MIC值明显较低.
光谱分析表明z-潜在和红外光谱的变化,表明相互作用和潜在的膜破坏.
该组合对真核细胞没有细胞毒性,同时影响了与铁进口和排泄相关的细菌基因表达.

结论:

联合Fe16+AMP疗法显示,对S. aureus有显著的协同作用和抗菌作用.
铁16增强了安皮西林的疗效,可能通过破坏细菌膜并改善药物吸收.
这种组合疗法作为打击抗生素耐药性的战略具有前景,需要进一步研究.

关键词:

阿姆皮西林是一种抗胰岛素.抗微生物活性的抗菌活性.铁复合体是一种铁复合体.黄金葡萄球菌黄金葡萄球菌协同效应是一种协同效应.

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