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抗消化乳清酸可促进Caco-2细胞的抗氧化作用

Juliana Santos de Espindola¹, Milena Ferreira Taccóla¹, Vera Sônia Nunes da Silva¹

¹Quality and Science Center of Food, Institute of Food Technology (ITAL), Brasil Ave. 2880, P.O. Box 139, Campinas, SP 13070-178, Brazil.

Food research international (Ottawa, Ont.)

|October 7, 2023

概括

消化过程中的乳清具有抗氧化作用,在没有额外的酶的情况下保护肠道细胞免受氧化应激. 这些维护胃肠道健康,对补充剂有好处.

科学领域:

食品科学食品科学食品科学
生物化学生物化学
细胞生物学细胞生物学

背景情况:

肠内皮细胞因摄入物质而面临氧化损伤.
乳清具有抗氧化潜力,但它们对肠道细胞和通路的影响尚不清楚.
卡科-2细胞作为研究肠道细胞反应的模型.

研究的目的:

为了评估乳清对模拟消化的抵抗力.
研究它们在Caco-2细胞中的抗氧化活性和机制.
为了将质结构与抗氧化功能相关联.

主要方法:

使用INFOGEST静态方法模拟胃肠道消化.
快速蛋白质液体染色学 (FPLC) 用于尺寸分析.
抗氧化剂测定 (ABTS,DPPH,ORAC) 和基于Caco-2细胞的测定 (ROS,GSH,SOD).

主要成果:

生成低分子量 (<600 Da) 乳清,具有增强的抗氧化活性 (蛋白质分离物的两倍).
确定了24个序列,富含疏水性和酸性氨基酸,与抗氧化剂相关的氨基酸 (lysine,valine,tyrosine).
证明乳清可以减弱活性氧物种,防止谷氨酸的减少,并维持Caco-2细胞中的超氧化物转化酶活性.

结论:

在模拟胃消化过程中产生的乳清酸具有固有的抗氧化特性.
这些可以保护胃肠道上皮细胞免受氧化应激.
不需要外源性酶预水解,这表明在消化健康补充剂中直接适用.

关键词:

抗氧化剂途径抗氧化剂途径抗氧化剂类是一种抗氧化剂.在Caco-2细胞中.信息报道信息报道乳清水解酸是乳清中的水解酸.

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