含有蛋白质纳米粒子的单体和双体乳液中黄蛋白的细分:对稳定性和生物可访问性的影响
Lauane Nunes1, Negin Hashemi2, Sandra Beyer Gregersen2
1CiFOOD, Food Science Department, Aarhus University, Aarhus N 9200, Denmark; Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, Campinas, São Paulo 13083-862, Brazil.
Food research international (Ottawa, Ont.)
|October 7, 2023
概括
蛋白质稳定乳液会影响黄蛋白的生物可访问性. 在双重乳液 (W-L/O/W) 的内部水相中封装黄蛋白增强了其体外消化,而单一乳液的生物可用性较低.
科学领域:
- 食品科学 食品科学 食品科学
- 营养科学 营养科学
- 材料科学 材料科学 材料科学
背景情况:
- 蛋白质稳定乳液是胡卜素的有效输送系统.
- 生物活性化合物在乳液中的结构安排会影响其疗效.
- 了解双重乳液中的黄蛋白局部化对于优化其生物可用性至关重要.
研究的目的:
- 研究蛋白在不同乳液结构中的定位对其稳定性和体外生物可访问性的影响.
- 为了比较水/油/水双乳液与单个油/水乳液在输送黄蛋白中的性能.
- 评估乳清蛋白分离物 (WPI) 纳米颗粒在蛋白封装中的作用.
主要方法:
- 制造水/油/水双乳液和油/水单乳液,在不同地点加入蛋白.
- 使用乳清蛋白分离物 (WPI) 纳米颗粒用于内相封装.
- 采用多糖醇聚里烯酸 (PGPR) 和桑坦作为稳定剂.
- 在模拟消化后评估蛋白对光的稳定性和体外生物可访问性.
主要成果:
- 在消化后,W-L/O/W双乳液在体外表现出最高的蛋白生物可访问性.
- 融入单一乳液 (O-L/W) 油相的氨酸显示生物可访问性明显较低.
- 在储存期间,W-L/O/W系统对光线暴露的稳定性降低.
- 双重乳液通常比单体乳液提供更高的黄蛋白生物可访问性.
结论:
- 蛋白质稳定乳液的结构设计显著影响蛋白的稳定性和生物可访问性.
- 在双重乳液的内水相内封装增强了氨酸的体外生物可用性.
- 优化乳液架构是开发有效的输送系统的关键,用于脂友生物活性化合物,如蛋白.
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