通过系统的优化促进Saccharomyces cerevisiae中cembratriene-ol的产生
Haiquan Yang1, Kunjie Zhang1, Wei Shen2
1The Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Biotechnology journal
|October 7, 2023
概括
生物降解生物杀虫剂cembratriene-ol可以在酵母中产生. 研究人员通过基因工程提高了Saccharomyces cerevisiae中的产量,实现了76.3倍的增加.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 可持续农业 可持续农业
背景情况:
- 坎布拉特-是一种单环双类化合物,在尼科蒂亚纳植物中合成.
- 它显示出作为可持续农业的可生物降解生物农药的潜力.
研究的目的:
- 在Saccharomyces cerevisiae中异质地表达cembratriene-ol合成的基因.
- 通过代谢工程策略优化cembratriene-ol的生产.
主要方法:
- 在S. cerevisiae中表达了geranylgeranyl二酸盐合成酶 (ggpps) 和cbts*Δp基因的异质表达.
- 在单个和双重促进体下,ERG20和cbts*Δp基因的过度表达.
- 合成基因的基因组集成和ERG9.9的动态控制.
- 关键酶的线粒体细分.
主要成果:
- 在工程化S. cerevisiae菌株中成功合成cembratriene-ol.
- 通过ERG20和cbts*Δp共同表达,提特增强高达1.84倍.
- 通过过度表达cbts*Δp和ggpps.获得的26.1倍的增加.
- 通过线粒体细分,cembratriene-ol标位显著增加了76.3倍.
结论:
- 糖菌的新陈代谢工程使其能够有效地生产cembratriene-ol.
- 系统的优化策略,包括基因表达和细分,对于高水平的产量至关重要.
- 这种方法对可持续的生物杀虫剂开发具有前景.
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