相关实验视频

Updated: Jul 14, 2025

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

无标签的定量热蛋白质组分析揭示了目标转录因子,其活动由MC3R信号调制

Friederike A Sandbaumhüter¹, Mariya Nezhyva¹, Per E Andrén^1,2

¹Department of Pharmaceutical Biosciences, Uppsala University, 751 24 Uppsala, Sweden.

Analytical chemistry

|October 7, 2023

概括

我们开发了一种用于热蛋白质组分析 (TPP) 的新工作流程,以分析在黑色皮质素3受体 (MC3R) 激活后的蛋白质变化和转录因子活性. 这种方法确定了参与细胞对MC3R信号反应的关键转录因子.

科学领域:

蛋白质组学是指蛋白质组学.
系统生物学系统生物学
分子信号传输的方法

背景情况:

使用无标签量化和离子移动性增强LC-MS的热蛋白质组概况 (TPP) 提供了丰富的数据集.
现有的分析方法可能无法完全整合差异表达,热稳定性和转录因子活性.
了解像MC3R这样的受体下游的信号通路对于细胞反应机制至关重要.

研究的目的:

开发和应用一个多维数据分析工作流程,用于无标签的定量TPP.
为了全面描述黑色皮质素3受体 (MC3R) 激活下游的信号传递过程.
识别差异表达的蛋白质,评估热稳定性,推断转录因子活动.

主要方法:

开发了一个多维数据分析工作流程,整合了基因组丰富,差异蛋白质表达和转录因子活动推断.
应用了无标签的定量热蛋白质组分析 (TPP) 与LC-MS相结合.
利用贝叶斯统计学来推断转录因子活性,并通过化的丰度进行验证.

主要成果:

鉴定了298种蛋白质,在MC3R激活时其热稳定性发生变化.
发现了关键的转录因子 (CCAR2,DDX21,HMGB2,SRSF7,TET2),其热稳定性发生变化,与免疫反应有关.
通过翻译后修改数据验证的推断转录因子活动,突出显示它们的调控作用.

结论:

开发的工作流程使得信号通路的全面表征成为可能.
提供了关于MC3R下游的蛋白质表达,热稳定性和转录因子调节的见解.
这些发现提供了对细胞对MC3R激活和相关免疫功能的反应的更深入的理解.

更多相关视频

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...