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用酶固定磁珠对单域抗体进行特定场所的修饰

Siyu Zhou¹, Xiaofeng Fang¹, Jiahui Lv¹

¹Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, Guangdong, China.

Bioconjugate chemistry

|October 7, 2023

概括

我们开发了带有固定微生物转质胺酶 (mTGase) 的磁珠,用于高效的纳米体修饰. 这种方法简化了净化,减少了蛋白质损失,并使向癌症成像和治疗成为可能.

科学领域:

生物结合化学生物结合化学
纳米技术纳米技术
生物医学工程生物医学工程

背景情况:

纳米体在癌症诊断和治疗方面具有前途.
使用微生物转胺酶 (mTGase) 的特定站点纳米体修饰面临着净化挑战.

研究的目的:

开发一个简单可靠的战略,用于特定站点的纳米体修饰.
为了改善纳米体生物结合物的蛋白质分离和净化过程.

主要方法:

在磁珠上固定mTGase (MB-mTGase).
使用磁性分离进行简化蛋白质净化.
纳米体与光染料和聚乙烯糖醇的特定位点结合.

主要成果:

MB-mTGase保留了高的催化活性,并证明了可重复使用性.
磁分离有效地净化了纳米体生物结合物,损失最小.
成功结合纳米体用于向免疫光成像,并改善了体内药理动力学.

结论:

MB-mTGase方法提供了高效,快速和成本效益的特定站点的纳米体功能化.
这种方法对推进基于纳米体的诊断和治疗有前途.
该方法简化了生物结合工作流程,并减少了材料损失.

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