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Updated: Jul 14, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

微生物组研究中的16S rRNA基因测序工作流程的批判性审查:从原始选择到高级数据分析

Alba Regueira-Iglesias¹, Carlos Balsa-Castro¹, Triana Blanco-Pintos¹

¹Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, A Coruña, Spain.

Molecular oral microbiology

|October 7, 2023

概括

本综述指导研究人员优化16S rRNA基因测序,用于口腔微生物群的研究. 它涵盖了原料选择,测序技术,数据分析和改进研究成果的先进方法.

科学领域:

微生物学和生物信息学
基因组学和分子生物学

背景情况:

16S核糖体核糖酸 (rRNA) 基因是微生物社区分析的关键基因标记,在公共存储库中拥有大量数据.
基因序列的多批次再分析越来越重要,特别是对于口腔微生物群,需要标准化的方法.

研究的目的:

为研究人员提供一本关于利用16S rRNA基因测序用于微生物群研究,特别是口腔微生物群的综合指南.
通过对原料选择,测序技术和生物信息学管道的最新知识来优化决策和增强研究结果.

主要方法:

综述16S rRNA基因作为遗传学标记物的优缺点.
对口腔微生物组研究中的多样性和分类学赋值的影响.
对16S rRNA基因数据处理的测序技术,平台和生物信息管道的评估.

主要成果:

确定了选择测序技术和平台的关键因素.
详细描述16S rRNA基因数据处理步骤和生物信息学管道选择.
关于微生物社区研究的先进分析方法,指数,指标和软件的概述.

结论:

未来的研究应该强调严格的分析方法,包括对健康和疾病中的基于微生物组的生物标志物的预测模型.
解决批量效应和实施微生物组特定的校正方法对于可靠的多批次再分析至关重要.

关键词:

16S rRNA 基因是16S 的基因.生物信息学是一种生物信息学.微生物组是一个微生物组.这是一个非常好的初级初级.测序的测序是指测序的测序.统计分析是一种统计分析.

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