相关实验视频

Updated: Jul 14, 2025

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Published on: September 10, 2021

在韩国本土的5种丝虫菌株的分子诊断中,使用从全基因组序列中选择的单核酸多态体

Jeong Sun Park¹, Seong-Wan Kim², Jong Woo Park²

¹Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.

Journal of insect science (Online)

|October 7, 2023

概括

通过分子诊断方法,准确识别了韩国五种特有丝虫菌株 (Bombyx mori). 这种方法利用单核酸多态 (SNP) 和决策树来在育种计划中可靠地区分菌株.

科学领域:

* 昆虫学和遗传学
* 分子生物学和基因组学

背景情况:

* 化丝虫 (Bombyx mori) 菌株鉴定是具有挑战性的,因为它们的形态和遗传相似性.
* 韩国有5种具有繁殖潜力的特有三变性丝虫菌株.
*新兴行业需求需要可靠的方法来识别这些优质菌株.

研究的目的:

* 建立一种分子诊断方法,用于识别5种特有三变性丝虫菌株.
* 开发一种基于决策树的方法,使用单核酸多态 (SNP).

主要方法:

*39个丝虫菌株的全基因组测序 (37个现有菌株,2个新菌株).
* 每个菌株选择 2-4 个单核酸多态 (SNP).
*使用SNP和四原放大阻燃突变系统-聚合酶链反应 (T-ARMS-PCR) 的决策树的开发,与PCR-限制片段长度多态化 (PCR-RFLP) 对于一个菌株.

主要成果:

* 基于决策树的分子方法在识别5种特有三变形丝虫菌株时实现了100%的准确性.
*T-ARMS-PCR成功分化了菌株,PCR-RFLP作为一种菌株的补充方法.
* 确定了许多额外的SNP,为未来的菌株诊断提供了潜力.

结论:

*开发的基于SNP的决策树方法为丝虫菌株识别提供了一致而准确的方法.
* 这种分子诊断工具通过准确识别有价值的特有丝虫菌株来支持育种计划.
*已识别的SNP可用于未来开发其他丝虫菌株的诊断工具.

关键词:

决策树模型决策树模型这是一种特有菌株.分子诊断分子诊断分子诊断丝虫是一种丝虫.一个核酸的多态性.

更多相关视频

Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles

Author Spotlight: Advancing Gene Silencing Research in Silkworms with dsRNA Delivery Through Feeding Chitosan Nanoparticles

Published on: October 4, 2024

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

Published on: March 28, 2025

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...