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Updated: Jul 14, 2025

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

使用修改后的微型皮皮特器选择单个人类多能干细胞的协议.

Sandra P Mojica-Perez¹, Kyle Stokes², Daniel C Jaklic²

¹Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA; The Human Stem Cell and Gene Editing Core, University of Michigan, Ann Arbor, MI 48109, USA.

|October 7, 2023

概括

这项研究提出了一个新的协议,用于隔离单个人类多能干细胞,使用重新设计的微操纵器. 这种方法有效地产生具有高存活率的同质克隆培养物,避免了细胞分类.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?神经科学是一个神经科学.干细胞是干细胞.

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Published on: November 20, 2016

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科学领域:

干细胞生物学干细胞生物学
生殖技术生殖技术

背景情况:

产生同质的人类多能干细胞 (hPSC) 培养物对于研究和治疗应用至关重要.
目前的单细胞隔离方法可能是劳动密集型或需要专门的设备,如细胞分类器.

研究的目的:

描述一种用于hPSCs单细胞克隆选择的新,可访问的协议.
为了适应通常用于体外受精的STRIPPER Micropipetter,以有效地隔离干细胞.

主要方法:

重新使用STRIPPER微管器来挑选单个hPSCs.
工具准备,单细胞隔离和殖民地通过的详细步骤.
随后进行细胞培养放大和分析的程序.

主要成果:

该协议成功生成了同质的克隆hPSC培养物.
在孤立的单细胞中实现了超过50%的生存率.
该方法不需要使用细胞分类.

结论:

重新设计的STRIPPER Micropipetter为hPSCs的单细胞克隆选择提供了一个实用和有效的替代方案.
该协议简化了统一干细胞种群的生成,提高了它们在各种研究领域的实用性.