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多隔间创伤导致持续的炎症和器官损伤
Jennifer A Munley1, Lauren S Kelly1, Gwendolyn S Gillies1
1Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida, College of Medicine, Gainesville, Florida.
The Journal of surgical research
|October 7, 2023
概括
鼠类多创伤会导致持续的全身炎症和器官功能障碍,持续至少一周. 雌性大鼠的脏恢复比雄性更好,突出了长期创伤模型研究的必要性.
科学领域:
- 创伤和伤害研究 创伤和伤害研究
- 系统性炎症 系统性炎症
- 多器官功能障碍 多器官功能障碍
背景情况:
- 之前的多部门损伤模型集中在短时间 (<72小时) 上.
- 多部门损伤的长期影响仍未确定.
- 这项研究调查了多创伤后的持续性炎症和器官功能障碍.
研究的目的:
- 建立和评估多部门损伤的临床前模型.
- 为了确定系统性炎症和多器官功能障碍是否在受伤后一周内持续存在.
- 为了比较雄性和雌性大鼠之间的长期结果.
主要方法:
- 雄性和雌性Sprague-Dawley大鼠接受了多创伤 (PT) 或作为天真对照.
- 在第2天和第7天评估体重,血红蛋白,血中性粒细胞凝酶相关性脂卡林 (NGAL) 和血收费类受体4 (TLR4)
- 在第7天组织学评估肺损伤,并将性别之间的功能进行比较.
主要成果:
- PT大鼠表现出显著的体重减轻和持续到第7天的贫血.
- 在两个时间点在PT大鼠中观察到血NGAL和TLR4的升高.
- 在PT大鼠的第7天显著的肺损伤是显而易见的,男性的功能比雌性更差.
结论:
- 多部门创伤会导致持续的全身炎症和多器官功能障碍.
- 与雄性相比,雌性大鼠的恢复得到改善.
- 在临床前模型中进行长期评估对于评估免疫调节疗法至关重要.
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