主要的神经毒素因子,Lansamide I,来自Clausena lansium水果和代谢功能障碍引发了代谢功能障碍
Juan Chen1, Xiaoyue Zhang2, Yuru Zhang1
1Shaanxi Academy of Traditional Chinese Medicine, Xi'an, 710003, Shaanxi, China.
概括
香种子含有兰沙胺I,一种有毒化合物,会导致斑马鱼的发育问题和神经损伤. 这一发现影响了这种传统草药和水果的安全使用.
科学领域:
- 植物化学 植物化学
- 毒理学 毒理学 毒理学
- 代谢学 代谢学 代谢学
背景情况:
- 旺皮 (Clausena lansium) 果肉是可食用的,而种子用于传统中医药.
- 在wampee种子中存在的和特定的有毒化合物并没有得到很好的描述.
- 了解wampee的毒性对于其安全消费和药用应用至关重要.
研究的目的:
- 为了识别和表征wampee种子中的主要有毒化合物.
- 为了研究这种化合物对斑马鱼的毒理作用.
- 为了阐明代谢反应和分子机制背后的wampee毒性.
主要方法:
- 使用核磁共振 (NMR) 和X射线衍射的化合物隔离和结构阐明.
- 基于表型的斑马鱼毒性评估,包括化能力,发育不良和发育迟缓.
- 对过氧化水平和乙胆酶活性进行生物化学测定.
- 代谢分析,以确定受影响的代谢途径.
- 定量实时聚合酶链反应 (qRT-PCR) 用于分析基因表达.
主要成果:
- 兰沙胺I被确定为wampee种子和水果中主要的有毒化合物.
- 兰沙胺I暴露导致斑马鱼化能力降低,形增加 (黄囊,心周),增长延迟.
- 该化合物诱导了神经细胞损伤,减少了运动活动,并增加了过氧化标记物 (甲,超氧化物脱酶).
- 代谢分析显示,氨基酸和参与核酸代谢和贝他因生物合成的显著上调.
- 显著改变了tat和dctpp基因的mRNA表达.
结论:
- 兰沙胺I是wampee中的关键毒剂,对斑马鱼观察到的发育和神经效应负责.
- 该化合物破坏细胞代谢,特别是氨基酸和核酸通路.
- 这些发现为wampee的毒性机制提供了关键的见解,并为其安全使用提供了信息.
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