使用新型生物涂层提高微藻-细菌生物膜中的有机物质生产率
C Y Tong1, Kohsuke Honda2, C J C Derek1
1School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Nibong Tebal, 14300 Penang, Malaysia.
The Science of the total environment
|October 7, 2023
概括
一种新的生物涂层在多孔基板上增强了微藻-细菌生物膜,促进了微藻生长和细菌繁殖. 这种共生方法增加了细胞内生物化学化合物,为生物化合物产量优化提供了一个新的策略.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 可再生能源可再生能源是可再生能源.
背景情况:
- 多孔基板光生物反应器对微藻可再生能源有兴趣,但仅限于纯种植.
- 了解微藻-细菌在微孔基板上的生物膜,特别是它们在有机物质丰富的环境中的相互相互作用,是一个研究挑战.
研究的目的:
- 在聚乙烯化物膜上使用微藻衍生的生物涂层建立一个新的培养平台.
- 使用这种生物涂层,研究微藻-细菌生物膜 (Chlorella vulgaris,Cylindrotheca fusiformis与大肠杆菌) 的增长增强.
主要方法:
- 开发了一种微藻衍生生物涂层,用于预先条件化水友膜.
- 在涂层和未涂层膜上的附着共培模式下培养的微藻-细菌生物膜.
- 量化微藻和细菌生长,以及细胞内生物化学化合物 (叶绿素,多糖,蛋白质,脂质).
主要成果:
- 生物涂层从第10天开始对两种物种增强了微藻的生长 (2.5%19%).
- 在适应后的共同培养中,生物涂层显著促进了细菌生长 (至少2.35倍).
- 由于预涂有机物,在五天内观察到细胞内生物化学化合物的显著增加 (≥72%).
结论:
- 微藻衍生的生物涂层有效地支持微藻-细菌生物膜的生长,并增强了生物质的关键成分.
- 生物涂层战略促进了共生相互作用,可能增加生物化合物产量.
- 这种方法为优化光生物反应器中的共生系统提供了一个范式转变.
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