在自然衰老小鼠的心脏中,蛋白质组学和β-基基化修饰特性
Xuechun Yang1, Xuehui Li1, Na Yu2
1Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Molecular & cellular proteomics : MCP
|October 7, 2023
概括
老化的心脏表现出改变的氨酸β-氧基化 (Kbhb) 模式,特别是在线粒体能量代谢蛋白中. 这项研究揭示了Kbhb作为与年龄有关的心血管疾病的潜在治疗点.
科学领域:
- 心血管生物学 心血管生物学
- 蛋白质组学是指蛋白质组学.
- 衰老研究研究 衰老研究
背景情况:
- 衰老是心血管疾病 (CVD) 的一个主要风险因素.
- lysine β-hydroxybutyrylation (Kbhb) 是一种新的翻译后修饰,在各种疾病中起作用.
- 在老年心脏中缺乏Kbhb的全球分析.
研究的目的:
- 研究老老鼠心中的全球蛋白质组和Kbhb修饰变化.
- 为了确定Kbhb修饰的特定蛋白质和受衰老影响的途径.
- 探索Kbhb作为心脏衰老和心血管疾病的潜在治疗点.
主要方法:
- 定量蛋白质组学和Kbhb后翻译修改组学在年轻和老老鼠心脏上进行.
- 使用高通量液态染色学和质谱学进行分析.
- 统计分析确定了差异修饰的Kbhb站点.
主要成果:
- 年龄较大的小鼠表现出较低的握力和心脏扩张功能.
- 在641种蛋白质中确定了1710个β-基基化氨酸位.
- 134个蛋白质中的183个Kbhb位点在老年心脏中显示出显著的差异性修饰.
- 在能量代谢途径和线粒体局部化方面,Kbhb修饰的蛋白质得到了丰富.
结论:
- 这项研究提供了第一个老年心肌细胞的全球蛋白质和Kbhb修饰景观.
- 老化心脏中Kbhb模式的改变与能量代谢功能障碍有关.
- 调节Kbhb修改为心脏衰老和心血管疾病提供了潜在的治疗策略.
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