在 BAF 染色体复合体中,SMARCC1 不会调解 GLI 转录抑制子的目标基因在肢体芽中的转录抑制
Janani Ramachandran1, Wanlu Chen2, Rachel K Lex3
1Department of Molecular Biosciences, University of Texas at Austin, 100 E 24th Street, Stop A5000, Austin, TX, 78712, USA.
Developmental biology
|October 7, 2023
概括
尽管在GLI增强剂中存在,但SMARCC1在小鼠四肢发育中的GLI抑制中并不起作用. 替代色素重塑复合物可能促进GLI抑制,影响的信号通路调节.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- (HH) 信号通路调节在小鼠四肢发育中至关重要.
- GLI蛋白质是介导HH通路反应的关键转录因子.
- 之前,BAF染色体重塑复合物与GLI抑制有关.
研究的目的:
- 调查核心BAF复合蛋白SMARCC1在GLI介导抑制中的作用.
- 为了确定SMARCC1是否对于肢体增强剂的GLI抑制是必要的.
主要方法:
- 对肢体增强剂的SMARCC1和GLI结合的分析.
- 评估GLI监管增强剂的染色质可访问性.
- 检查Smarcc1条件淘汰赛小鼠四肢中的基因表达.
- 调查子路径突变体中的GLI抑制.
主要成果:
- 在大多数活跃的肢体增强剂中存在SMARCC1,包括那些与GLI结合的.
- 在这些增强剂中,SMARCC1维持,而不是减少染色质的可访问性.
- SMARCC1 的结合是独立于 GLI3.3.
- 大多数GLI目标基因在Smarcc1淘汰中不受影响.
- 在HH突变肢体芽中,SMARCC1不需要用于GLI抑制.
结论:
- 不过,SMARCC1并不是GLI3压制的媒介.
- 替代色素重塑复合物可能参与GLI抑制.
- 在肢体发育中SMARCC1的作用可能包括保持增强器的可访问性,而不是直接抑制GLI.
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